Activation of p53 Gene Expression and Synergistic Antiproliferative Effects of 5-Fluorouracil and B-escin on MCF7 Cells

Raziyeh Mazrouei, Elham Raeisi, Yves Lemoigne, Esfandiar Heidarian

DOI: 10.4103/jmss.JMSS_44_18

Abstract


One of the most common malignancies in women is breast cancer. B-escin has pharmacological anticancer effects. 5-fluorouracil (5-FU) has antimetabolite and antiproliferative properties. The purpose of this study was to investigate the combined effects of 5-FU and B-escin on apoptosis, colony formation, Bcl-2 signaling protein, and p53 gene expression in MCF7 breast cancer cell line. The cytotoxic effects, the number of colonies, apoptosis, p53 gene expression, and Bcl-2 signaling protein of the combined 5-FU and B-escin on MCF7 cells were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and western blotting methods, respectively. Half-maximal inhibitory concentration values of B-escin and 5-FU were 80 Micro g/ml and 2 Micro M, respectively. The combination of 5-FU and B-escin on MCF7 cell viability showed a combination index equal to 0.5. The expression of p53 and apoptosis increased in the combination of 5-FU and B-escin on MCF7 cells compared to that of control group (P < 0.05). In addition, the number of colonies and Bcl-2 signaling protein in combination of 5-FU and B-escin decreased with respect to untreated control cells or single treatment of 5-FU and B-escin. The combination of 5-FU and B-escin not only has synergistic effects by increasing cell apoptosis and p53 gene expression but also decreases Bcl-2 signaling protein in MCF7 cell lines. 


Keywords


5-fluorouracil, apoptosis, MCF7, p53, B-escin

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