Evaluation of Mechanical Properties and Cell Viability of Poly (3-Hydroxybutyrate)-Chitosan/Al2O3 Nanocomposite Scaffold for Cartilage Tissue Engineering
DOI: 10.4103/jmss.JMSS_56_18
Abstract
Background: The aim of this study was to evaluate the effects of alumina nanowires as reinforcement phases in polyhydroxybutyrate-chitosan (PHB-CTS) scaffolds to apply in cartilage tissue engineering. Methods: A certain proportion of polymers and alumina was chosen. After optimization of electrospun parameters, PHB, PHB-CTS, and PHB-CTS/3% Al2O3 nanocomposite scaffolds were randomly electrospun. Scanning electron microscopy, Fourier transform infrared spectroscopy, water contact angle measurement, tensile strength, and chondrocyte cell culture studies were used to evaluate the physical, mechanical, and biological properties of the scaffolds. Results: The average fiber diameter of scaffolds was 300-550 nm and the porosity percentages for the first layer of all types of scaffolds were more than 81%. Scaffolds' hydrophilicity was increased by adding alumina and CTS. The tensile strength of scaffolds decreased by adding CTS and increased up to more than 10 folds after adding alumina. Chondrocyte viability and proliferation on scaffolds were better after adding CTS and alumina to PHB. Conclusion: With regard to the results, electrospun PHB-CTS/3% Al2O3 scaffold has the appropriate potential to apply in cartilage tissue engineering.
Keywords
References
Roseti L, Parisi V, Petretta M, Cavallo C, Desando G, Bartolotti I, et al. Scaffolds for bone tissue engineering: State of the art and new perspectives. Mater Sci Eng C Mater Biol Appl 2017;78:1246-62.
Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. In: The Biomaterials: Silver Jubilee Compendium. Elsevier Science, 2000. p. 175-89.
Sadeghi D, Karbasi S, Razavi S, Mohammadi S, Shokrgozar MA, Bonakdar S. Electrospun poly (hydroxybutyrate)/chitosan blend fibrous scaffolds for cartilage tissue engineering. Journal of Applied Polymer Science, 2016;133:47.
Karbasi S, Alizadeh ZM. Effects of multi-wall carbon nanotubes on structural and mechanical properties of poly (3hydroxybutyrate)/chitosan electrospun scaffolds for cartilage tissue engineering. Bull Mater Sci 2017;40:1247-53.
Khoroushi M, Foroughi MR, Karbasi S, Hashemibeni B, Khademi AA. Effect of polyhydroxybutyrate/chitosan/bioglass nanofiber scaffold on proliferation and differentiation of stem cells from human exfoliated deciduous teeth into odontoblast-like cells. Mater Sci Eng C Mater Biol Appl 2018;89:128-39.
Karbasi S, Zarei M, Foroughi M. Effects of multi-wall carbon nano-tubes (MWNTs) on structural and mechanical properties of electrospun poly (3-hydroxybutyrate) scaffold for tissue engineering applications. Sci Iranica Trans F Nanotechnol 2016;23:3145.
Alzarrug FA, Dimitrijevic MM, Heinemann RM, Radojevic V, Stojanovic DB, Uskokovic PS, et al. The use of different alumina fillers for improvement of the mechanical properties of hybrid PMMA composites. Mater Des 2015;86:575-81.
Fu SY, Lauke B. The elastic modulus of misaligned short-fiber-reinforced polymers. Compos Sci Technol 1998;58:389-400.
Li D, Xia Y. Electrospinning of nanofibers: Reinventing the wheel? Adv Mater 2004;16:1151-70.
Dong Z, Wu Y, Wang Q, Xie C, Ren Y, Clark RL, et al. Reinforcement of electrospun membranes using nanoscale al2O3 whiskers for improved tissue scaffolds. J Biomed Mater Res A 2012;100:903-10.
Song Y, Ju Y, Song G, Morita Y.In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina. Int J Nanomedicine 2013;8:2745-56.
Karlsson M, Palsgard E, Wilshaw PR, Di Silvio L. Initialin vitro interaction of osteoblasts with nano-porous alumina. Biomaterials 2003;24:3039-46.
Hashimoto M, Sasaki J, Imazato S. Investigation of the cytotoxicity of aluminum oxide nanoparticles and nanowires and their localization in L929 fibroblasts and RAW264 macrophages. J Biomed Mater Res B Appl Biomater 2016;104:241-52.
Asran AS, Razghandi K, Aggarwal N, Michler GH, Groth T. Nanofibers from blends of polyvinyl alcohol and polyhydroxy butyrate as potential scaffold material for tissue engineering of skin. Biomacromolecules 2010;11:3413-21.
Naveen N, Kumar R, Balaji S, Uma T, Natrajan T, Sehgal P. Synthesis of nonwoven nanofibers by electrospinning – A promising biomaterial for tissue engineering and drug delivery. Adv Eng Materials 2010;12:B380-7.
Zhijiang C, Yi X, Haizheng Y, Jia J, Liu Y. Poly (hydroxybutyrate)/cellulose acetate blend nanofiber scaffolds: Preparation, characterization and cytocompatibility. Mater Sci Eng C Mater Biol Appl 2016;58:757-67.
Medvecky L, Giretova M, Stulajterova R. Properties andin vitro characterization of polyhydroxybutyrate-chitosan scaffolds prepared by modified precipitation method. J Mater Sci Mater Med 2014;25:777-89.
Ghasemi-Mobarakeh L, Semnani D, Morshed M. A novel method for porosity measurement of various surface layers of nanofibers mat using image analysis for tissue engineering applications. J Applied Polym Sci 2007;106:2536-42.
Rodríguez-Lorenzo LM, Salinas AJ, Vallet-Regí M, San Román J. Composite biomaterials based on ceramic polymers. I. Reinforced systems based on Al2O3/PMMA/PLLA. J Biomed Mater Res 1996;30:515-22.
Patel AK, Balani K. Dispersion fraction enhances cellular growth of carbon nanotube and aluminum oxide reinforced ultrahigh molecular weight polyethylene biocomposites. Mater Sci Eng C Mater Biol Appl 2015;46:504-13.
Bagheripour E, Moghadassi A, Hosseini S, Van der Bruggen B, Parvizian F. Novel composite graphene oxide/chitosan nanoplates incorporated into PES based nanofiltration membrane: Chromium removal and antifouling enhancement. J Ind Eng Chem 2018;62:311-20.
Ishak NF, Hashim NA, Othman MH, Monash P, Zuki FM. Recent progress in the hydrophilic modification of alumina membranes for protein separation and purification. Ceram Int 2017;43:915-25.
Thambyah A, Nather A, Goh J. Mechanical properties of articular cartilage covered by the meniscus. Osteoarthritis Cartilage 2006;14:580-8.
Nagiah N, Madhavi L, Anitha R, Srinivasan NT, Sivagnanam UT. Electrospinning of poly (3-hydroxybutyric acid) and gelatin blended thin films: Fabrication, characterization, and application in skin regeneration. Polym Bull 2013;70:2337-58.
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