Effects of Nano-biphasic Calcium Phosphate Composite on Bioactivity and Osteoblast Cell Behavior in Tissue Engineering Applications

Mehdi Ebrahimian Hosseinabadi, Mohammadreza Etemadifar, Fakhredin Ashrafizadeh

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Abstract


In this paper, preparation, bioactivity, and osteoblast cell behavior of cortical bone derived nanobiphasic calcium phosphate (nano-BCP) are presented. The calcined bovine bone samples with the addition of di-ammonium hydrogen phosphate were heated at 700°C for 100 min, and thus nano-BCP with the composition of 63/37 hydroxyapatite (HA)/β-tricalcium phosphate (β-TCP) was produced. Scanning electron microscopy (SEM) images, energy dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) analysis of immersed samples in simulated body fluid (SBF) solution showed that a uniform layer was formed on the surface after 7 days with the chemical composition of HA. The results indicated that the nano-BCP sample developed excellent bioactivity after 28 days. The nano-BCP samples showed better cell proliferation compared to pure HA samples. After 7 days in cell culture, the prepared nano-BCP (HA/β-TCP) exhibited the maximum proliferation of the MG-63 osteoblast cells.

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